Journal: Translational Oncology

Article Title: Anticancer Activity of a Broccoli Derivative, Sulforaphane, in Barrett Adenocarcinoma: Potential Use in Chemoprevention and as Adjuvant in Chemotherapy 1

doi:

Figure Lengend Snippet: Effect of SFN on BEAC cell survival. BEAC cells were cultured in the medium containing no SFN or various concentrations of SFN. Cells were harvested at different time points as indicated and proliferative potential was assessed by trypan blue exclusion and/or proliferation assay, based on the production of a yellow product (formazan) after reduction of a highly water-soluble tetrazolium salt by dehydrogenases in viable cells. The growth curves show the mean of three independent experiments, with SEM. (A) Barrett adenocarcinoma (FLO-1) cells treated with various concentrations of SFN. (B) BEAC (OE33) cells treated with various concentrations of SFN. (C) Photomicrograph of BEAC (FLO-1 and OE33) cells treated with 3 µM SFN for 72 hours. (D) Photomicrograph of normal diploid fibroblasts and primary normal esophageal epithelial cells (ScienCell Research Laboratories) treated with 3 µM SFN for 72 hours. (E) FLO-1 cells were treated with SFN for 48 hours, detached floating cells from the medium and the attached cells (by trypsinization) were collected separately and evaluated for number and viability using trypan blue exclusion. The number of cells detached after treatment with various concentrations of SFN is expressed as percent of untreated FLO-1 cells. “Total” represents the total number of detached cells whereas “Dead” reflects the fraction of dead cells in detached cell population. (F) Panel (I): FLO-1 cells were incubated with various concentrations of SFN for 48 hours, and the expression of caspase 8 was detected by Western blot analysis, using anti-caspase 8 mouse monoclonal antibody (Cell Signaling, Danvers, MA). Panel (II): Bar graph showing caspase 8 expression relative to β-actin.

Article Snippet: Normal primary human esophageal epithelial cells (HEEC) were purchased from ScienCell Research Laboratories (Carlsbad, CA) and have been described previously [ 30 ].

Techniques: Cell Culture, Proliferation Assay, Incubation, Expressing, Western Blot

Journal: Heliyon

Article Title: Cell cycle associated protein 1 associates with immune infiltration and ferroptosis in gastrointestinal cancer

doi: 10.1016/j.heliyon.2024.e28794

Figure Lengend Snippet: The expression levels of CAPRIN1 and immune cells markers in gastrointestinal cancer cells and ESCA tumor tissues. (A–B) CAPRIN1, MHC-1, Shn2, CD45 proteins were expressed in different cells, including HEK239T, liver cancer cells (HepG2 and MHCC-97H), Hela, colon cancer cells (HCT116, SW480 and HT29), normal squamous epithelial cell line HET1A, ESCA cells (KYSE150, KYSE30). (C–D) Western blot analysis showing the expression of CAPRIN1 and MHC-1 in KYSE30, KYSE150, and MHCC-97H cells following CAPRIN1 knockdown. (E) Representative immunofluorescence images showing CAPRIN1 and MHC-1 expression levels following stable CAPRIN1 knockdown in KYSE150 cells. (F) The expression level of different immune markers (MHC-1, CD11b, CD45, and PD-L1) as determined by Western blotting in 14 paired ESCA tissues and adjacent tissues. T, ESCA tissues; N, paired adjacent normal tissues. * P < 0.05.

Article Snippet: Human cell lines, HEK239T, liver cancer cells (HepG2 and MHCC-97H), Hela, colon cancer cells (HCT116, SW480, and HT29), normal squamous epithelial cell line HET1A, ESCA cells (KYSE150, KYSE30) were purchased from iCell Bioscience Inc (China).

Techniques: Expressing, Western Blot, Knockdown, Immunofluorescence